In humans, trillions of bacteria live within this space. Most of these gut bacteria are beneficial, but pathogenic bacteria can also colonize the gastrointestinal tract, causing serious and potentially fatal infections. Because gut bacteria are significantly affected by their environment, the research team wondered whether the serotonin produced in the gut can affect the virulence of pathogenic bacteria that infect the gastrointestinal tract.
They worked with Escherichia coli O157, a species of bacteria that causes periodic outbreaks of often deadly foodborne infection. The team grew these pathogenic bacteria in Petri dishes in the lab, then exposed them to serotonin. Additional experiments using human cells showed that the bacteria could no longer cause infection-associated lesions on the cells if these bacteria were exposed to serotonin. Next, the researchers examined how serotonin affected virulence in living hosts.
Using mice, the researchers studied how serotonin might change the ability for Citrobacter rodentium a mouse gut bacterium often used as an analog for E. coli in humans to infect and sicken their hosts. These mice were genetically modified to either over or under produce serotonin in their gastrointestinal tracts. Always women does more jobs and so they lose their enerygy levels and become a tension full mankind.
